dominant-negative form of Search Results


myc tcf dominant negative  (Addgene inc)
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Addgene inc myc tcf dominant negative
Myc Tcf Dominant Negative, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dominant-negative form of dynamin  (Schmid GmbH)
90
Schmid GmbH dominant-negative form of dynamin
Dominant Negative Form Of Dynamin, supplied by Schmid GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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adenoviral expression vectors containing the dominant negative (dn) form of camkkβ (1.01 × 10 10 pfu/ml)  (SignaGen)
90
SignaGen adenoviral expression vectors containing the dominant negative (dn) form of camkkβ (1.01 × 10 10 pfu/ml)
Adenoviral Expression Vectors Containing The Dominant Negative (Dn) Form Of Camkkβ (1.01 × 10 10 Pfu/Ml), supplied by SignaGen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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adenoviral expression vectors containing the dominant negative (dn) form of camkkβ (1.01 × 10 10 pfu/ml) - by Bioz Stars, 2026-09
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cdc42 signaling molecule  (SLIT2 LTD)
90
SLIT2 LTD cdc42 signaling molecule
Slit2 inhibits <t>Cdc42</t> activity through srGAP1. a Purification of GST-PAK1 protein. GST-PAK1 fusion proteins were expressed in E. coli (BL21 strain) and purified using GST-sepharose beads. M: protein marker; Total protein from control BL21 strain(1) and BL21 strain expressing GST-PAK1(2); 3: Purified GST-PAK1 protein. b Slit2 inhibited Cdc42 activity in a Robo-dependent manner. Ectopic expression of Slit2 inhibited Cdc42 activity (Cdc42-GTP) in LoVo cells, which could be blocked by ectopic expression of RoboN. GTP-loading of Cdc42 (GTP-Cdc42) in LoVo cell extracts was pulled down using GST-sepharose beads loaded with GST-PAK1 protein and was detected by Western blot using an anti-Cdc42 antibody. c Slit2 inhibited Cdc42 activity through srGAP1. Ectopic expression of srGAP1 enhanced the inhibitory effect of Slit2 on the GTPase of Cdc42, whereas blocking srGAP1 activity by srGAP1ΔGAP rescued the Cdc42-GTP levels in Slit2-overexpressed LoVo cells
Cdc42 Signaling Molecule, supplied by SLIT2 LTD, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cdc42 signaling molecule - by Bioz Stars, 2026-09
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myc-his–tagged dominant-negative form of akt (k179m) in the puseamp plasmid  (United Biotech Inc)
90
United Biotech Inc myc-his–tagged dominant-negative form of akt (k179m) in the puseamp plasmid
Slit2 inhibits <t>Cdc42</t> activity through srGAP1. a Purification of GST-PAK1 protein. GST-PAK1 fusion proteins were expressed in E. coli (BL21 strain) and purified using GST-sepharose beads. M: protein marker; Total protein from control BL21 strain(1) and BL21 strain expressing GST-PAK1(2); 3: Purified GST-PAK1 protein. b Slit2 inhibited Cdc42 activity in a Robo-dependent manner. Ectopic expression of Slit2 inhibited Cdc42 activity (Cdc42-GTP) in LoVo cells, which could be blocked by ectopic expression of RoboN. GTP-loading of Cdc42 (GTP-Cdc42) in LoVo cell extracts was pulled down using GST-sepharose beads loaded with GST-PAK1 protein and was detected by Western blot using an anti-Cdc42 antibody. c Slit2 inhibited Cdc42 activity through srGAP1. Ectopic expression of srGAP1 enhanced the inhibitory effect of Slit2 on the GTPase of Cdc42, whereas blocking srGAP1 activity by srGAP1ΔGAP rescued the Cdc42-GTP levels in Slit2-overexpressed LoVo cells
Myc His–Tagged Dominant Negative Form Of Akt (K179m) In The Puseamp Plasmid, supplied by United Biotech Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plasmids encoding a dominant negative form of ras (rasn17)  (Johns Hopkins HealthCare)
90
Johns Hopkins HealthCare plasmids encoding a dominant negative form of ras (rasn17)
Slit2 inhibits <t>Cdc42</t> activity through srGAP1. a Purification of GST-PAK1 protein. GST-PAK1 fusion proteins were expressed in E. coli (BL21 strain) and purified using GST-sepharose beads. M: protein marker; Total protein from control BL21 strain(1) and BL21 strain expressing GST-PAK1(2); 3: Purified GST-PAK1 protein. b Slit2 inhibited Cdc42 activity in a Robo-dependent manner. Ectopic expression of Slit2 inhibited Cdc42 activity (Cdc42-GTP) in LoVo cells, which could be blocked by ectopic expression of RoboN. GTP-loading of Cdc42 (GTP-Cdc42) in LoVo cell extracts was pulled down using GST-sepharose beads loaded with GST-PAK1 protein and was detected by Western blot using an anti-Cdc42 antibody. c Slit2 inhibited Cdc42 activity through srGAP1. Ectopic expression of srGAP1 enhanced the inhibitory effect of Slit2 on the GTPase of Cdc42, whereas blocking srGAP1 activity by srGAP1ΔGAP rescued the Cdc42-GTP levels in Slit2-overexpressed LoVo cells
Plasmids Encoding A Dominant Negative Form Of Ras (Rasn17), supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dominant-negative+form+of/plasmids+encoding+a+dominant+negative+form+of+ras++rasn17+/10__1074_slash_jbc__m405164200-58-7-19
Average 90 stars, based on 1 article reviews
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dominant negative form of ras  (Johns Hopkins HealthCare)
90
Johns Hopkins HealthCare dominant negative form of ras
Slit2 inhibits <t>Cdc42</t> activity through srGAP1. a Purification of GST-PAK1 protein. GST-PAK1 fusion proteins were expressed in E. coli (BL21 strain) and purified using GST-sepharose beads. M: protein marker; Total protein from control BL21 strain(1) and BL21 strain expressing GST-PAK1(2); 3: Purified GST-PAK1 protein. b Slit2 inhibited Cdc42 activity in a Robo-dependent manner. Ectopic expression of Slit2 inhibited Cdc42 activity (Cdc42-GTP) in LoVo cells, which could be blocked by ectopic expression of RoboN. GTP-loading of Cdc42 (GTP-Cdc42) in LoVo cell extracts was pulled down using GST-sepharose beads loaded with GST-PAK1 protein and was detected by Western blot using an anti-Cdc42 antibody. c Slit2 inhibited Cdc42 activity through srGAP1. Ectopic expression of srGAP1 enhanced the inhibitory effect of Slit2 on the GTPase of Cdc42, whereas blocking srGAP1 activity by srGAP1ΔGAP rescued the Cdc42-GTP levels in Slit2-overexpressed LoVo cells
Dominant Negative Form Of Ras, supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dominant-negative+form+of/dominant+negative+form+of+ras/10__1074_slash_jbc__m308197200-60-7-18
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dominant negative form of ras - by Bioz Stars, 2026-09
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dominant-negative form of ras  (Schmid GmbH)
90
Schmid GmbH dominant-negative form of ras
Slit2 inhibits <t>Cdc42</t> activity through srGAP1. a Purification of GST-PAK1 protein. GST-PAK1 fusion proteins were expressed in E. coli (BL21 strain) and purified using GST-sepharose beads. M: protein marker; Total protein from control BL21 strain(1) and BL21 strain expressing GST-PAK1(2); 3: Purified GST-PAK1 protein. b Slit2 inhibited Cdc42 activity in a Robo-dependent manner. Ectopic expression of Slit2 inhibited Cdc42 activity (Cdc42-GTP) in LoVo cells, which could be blocked by ectopic expression of RoboN. GTP-loading of Cdc42 (GTP-Cdc42) in LoVo cell extracts was pulled down using GST-sepharose beads loaded with GST-PAK1 protein and was detected by Western blot using an anti-Cdc42 antibody. c Slit2 inhibited Cdc42 activity through srGAP1. Ectopic expression of srGAP1 enhanced the inhibitory effect of Slit2 on the GTPase of Cdc42, whereas blocking srGAP1 activity by srGAP1ΔGAP rescued the Cdc42-GTP levels in Slit2-overexpressed LoVo cells
Dominant Negative Form Of Ras, supplied by Schmid GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dominant-negative+form+of/dominant+negative+form+of+ras/pm17975827-234-20-35
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overexpression of a dominant-negative form of trkb  (Federation of European Neuroscience Societies)
90
Federation of European Neuroscience Societies overexpression of a dominant-negative form of trkb
Slit2 inhibits <t>Cdc42</t> activity through srGAP1. a Purification of GST-PAK1 protein. GST-PAK1 fusion proteins were expressed in E. coli (BL21 strain) and purified using GST-sepharose beads. M: protein marker; Total protein from control BL21 strain(1) and BL21 strain expressing GST-PAK1(2); 3: Purified GST-PAK1 protein. b Slit2 inhibited Cdc42 activity in a Robo-dependent manner. Ectopic expression of Slit2 inhibited Cdc42 activity (Cdc42-GTP) in LoVo cells, which could be blocked by ectopic expression of RoboN. GTP-loading of Cdc42 (GTP-Cdc42) in LoVo cell extracts was pulled down using GST-sepharose beads loaded with GST-PAK1 protein and was detected by Western blot using an anti-Cdc42 antibody. c Slit2 inhibited Cdc42 activity through srGAP1. Ectopic expression of srGAP1 enhanced the inhibitory effect of Slit2 on the GTPase of Cdc42, whereas blocking srGAP1 activity by srGAP1ΔGAP rescued the Cdc42-GTP levels in Slit2-overexpressed LoVo cells
Overexpression Of A Dominant Negative Form Of Trkb, supplied by Federation of European Neuroscience Societies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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overexpression of a dominant-negative form of trkb - by Bioz Stars, 2026-09
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Slit2 inhibits Cdc42 activity through srGAP1. a Purification of GST-PAK1 protein. GST-PAK1 fusion proteins were expressed in E. coli (BL21 strain) and purified using GST-sepharose beads. M: protein marker; Total protein from control BL21 strain(1) and BL21 strain expressing GST-PAK1(2); 3: Purified GST-PAK1 protein. b Slit2 inhibited Cdc42 activity in a Robo-dependent manner. Ectopic expression of Slit2 inhibited Cdc42 activity (Cdc42-GTP) in LoVo cells, which could be blocked by ectopic expression of RoboN. GTP-loading of Cdc42 (GTP-Cdc42) in LoVo cell extracts was pulled down using GST-sepharose beads loaded with GST-PAK1 protein and was detected by Western blot using an anti-Cdc42 antibody. c Slit2 inhibited Cdc42 activity through srGAP1. Ectopic expression of srGAP1 enhanced the inhibitory effect of Slit2 on the GTPase of Cdc42, whereas blocking srGAP1 activity by srGAP1ΔGAP rescued the Cdc42-GTP levels in Slit2-overexpressed LoVo cells

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: srGAP1 mediates the migration inhibition effect of Slit2-Robo1 in colorectal cancer

doi: 10.1186/s13046-016-0469-x

Figure Lengend Snippet: Slit2 inhibits Cdc42 activity through srGAP1. a Purification of GST-PAK1 protein. GST-PAK1 fusion proteins were expressed in E. coli (BL21 strain) and purified using GST-sepharose beads. M: protein marker; Total protein from control BL21 strain(1) and BL21 strain expressing GST-PAK1(2); 3: Purified GST-PAK1 protein. b Slit2 inhibited Cdc42 activity in a Robo-dependent manner. Ectopic expression of Slit2 inhibited Cdc42 activity (Cdc42-GTP) in LoVo cells, which could be blocked by ectopic expression of RoboN. GTP-loading of Cdc42 (GTP-Cdc42) in LoVo cell extracts was pulled down using GST-sepharose beads loaded with GST-PAK1 protein and was detected by Western blot using an anti-Cdc42 antibody. c Slit2 inhibited Cdc42 activity through srGAP1. Ectopic expression of srGAP1 enhanced the inhibitory effect of Slit2 on the GTPase of Cdc42, whereas blocking srGAP1 activity by srGAP1ΔGAP rescued the Cdc42-GTP levels in Slit2-overexpressed LoVo cells

Article Snippet: When Robo1 or srGAP1 activity was blocked, the CDC42 activity was rescued in Slit2-treated CRC cells.

Techniques: Activity Assay, Purification, Marker, Control, Expressing, Western Blot, Blocking Assay

srGAP1 mediates the migration inhibitory function of Slit2 by inhibiting Cdc42 activity in CRC cells. a-b Slit2 inhibits CRC motility through srGAP1. Ectopic expression of srGAP1 enhanced the inhibitory effect of Slit2 on the migration of LoVo cells, whereas blocking the srGAP1 activity by srGAP1ΔGAP rescued the migration ability of Slit-treated LoVo cells. c-d Cdc42 is a major functional target of the Slit2-Robo1 signaling in inhibiting CRC cell migration. Blocking Cdc42 activity using DNCdc42 (dominant negative) mimicked the inhibitory effect of Slit2 on the CRC cell migration, whereas ectopic expression of CACdc42 (constitutively active) retrieved the decreased cell motility by Slit2 treatment. * P < 0.001; ** P < 0.0001

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: srGAP1 mediates the migration inhibition effect of Slit2-Robo1 in colorectal cancer

doi: 10.1186/s13046-016-0469-x

Figure Lengend Snippet: srGAP1 mediates the migration inhibitory function of Slit2 by inhibiting Cdc42 activity in CRC cells. a-b Slit2 inhibits CRC motility through srGAP1. Ectopic expression of srGAP1 enhanced the inhibitory effect of Slit2 on the migration of LoVo cells, whereas blocking the srGAP1 activity by srGAP1ΔGAP rescued the migration ability of Slit-treated LoVo cells. c-d Cdc42 is a major functional target of the Slit2-Robo1 signaling in inhibiting CRC cell migration. Blocking Cdc42 activity using DNCdc42 (dominant negative) mimicked the inhibitory effect of Slit2 on the CRC cell migration, whereas ectopic expression of CACdc42 (constitutively active) retrieved the decreased cell motility by Slit2 treatment. * P < 0.001; ** P < 0.0001

Article Snippet: When Robo1 or srGAP1 activity was blocked, the CDC42 activity was rescued in Slit2-treated CRC cells.

Techniques: Migration, Activity Assay, Expressing, Blocking Assay, Functional Assay, Dominant Negative Mutation

Schematic diagram of the mechanism by which srGAP1 mediating the tumor-suppressive activity of Slit2 in CRC. Slit2 inhibits CRC cell migration through the srGAP1-Cdc42 axis, and USP33 is required for the inhibitory function of Slit2-Robo1 by deubiquitinating and stabilizing Robo1 as well as taking part in the redistribution of Robo1 to the plasma membrane stimulated by Slit2

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: srGAP1 mediates the migration inhibition effect of Slit2-Robo1 in colorectal cancer

doi: 10.1186/s13046-016-0469-x

Figure Lengend Snippet: Schematic diagram of the mechanism by which srGAP1 mediating the tumor-suppressive activity of Slit2 in CRC. Slit2 inhibits CRC cell migration through the srGAP1-Cdc42 axis, and USP33 is required for the inhibitory function of Slit2-Robo1 by deubiquitinating and stabilizing Robo1 as well as taking part in the redistribution of Robo1 to the plasma membrane stimulated by Slit2

Article Snippet: When Robo1 or srGAP1 activity was blocked, the CDC42 activity was rescued in Slit2-treated CRC cells.

Techniques: Activity Assay, Migration, Clinical Proteomics, Membrane